MSCs Isolation & Expansion

MSCs can be derived from different sources and can be isolated from various tissues, such as umbilical cord, bone marrow, cartilage, muscle, and adipose tissues. Besides, fetal dermis tissues, various dental tissues, pancreatic tissue, breast milk, menstrual blood, and endometrium have been reported to contain MSCs as well. Additionally, the MSC pool of a tissue may be expanded by dedifferentiation of differentiated cells under certain conditions. Cells from different tissues differ in properties including cell surface markers expressed and differentiation abilities. Even populations derived from a single source may be heterogeneous and have different developmental potentials.

Creative Biolabs employs different protocols for MSC isolation.

• Firstly, the adherence properties of MSCs can be utilized for simple isolation of MSCs. MSCs are obtained by seeding the bone marrow cells on tissue plastic dishes, followed by washing out of the non-adherent cells.
• Secondly, the extraction of the mononuclear cells from the bone marrow through density gradient centrifugation or the enzymatic treatment of solid tissues with collagenase can be used to increase the yield of MSCs.
• Besides, the immunoisolation and immunodepletion techniques based on different surface markers, in combination with fluorescence-activated cell sorting or magnetic beads, can improve the purity of the resulted MSCs yields.

Learn more: bone marrow stem cells isolation